Anti-MAP2; clone AP20; Biotin Conjugate

Application

Anti-MAP2 Antibody, clone AP20, Biotin Conjugated is an antibody against MAP2 for use in ICC.

Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected MAP2 in rat E18 primary cortex cells.

General description

The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin, stabilizes microtubules (Herzog and Weber, 1978), and is an excellent marker for neurons. MAP-2 is essential for the development and maintenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurours. (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules.

Immunogen

Bovine brain microtubule protein

Physical form

Purified mouse monoclonal IgG conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Immunohistochemistry in rat cortex tissue.

Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected MAP2 in rat cortex tissue.

Target description

The unconjugated parent antibody (Cat. No. MAB3418) has an observed molecular weight at ~230 kDa